SARS-CoV-2-like Spike Protein Sequences with Affinity to hACE2 Were Present in the Public Databases in 2005. Plus, a Hint on SARS-CoV-2 Laboratory Origin
SARS-CoV-2-like Spike Protein Sequences with Affinity to hACE2 Were Present in the Public Databases in 2005. Plus, a Hint on SARS-CoV-2 Laboratory Origin
Lau et al. published three sequences with an interesting legacy that involves genetic manipulation by Ralph Baric published in 2008. The implications are profound; Why is everyone ignoring it?
This is great. Skeptics of contamination sources effecting SARS-CoV-2 genome alignment & assembly have access to the read-level data and can fund their own bioinformatics analysis to back their claims of arbitrariness. De novo sequence assembly by independent teams using no reference for alignment should be sufficient evidence to convince them. https://registry.opendata.aws/ncbi-sra/
Absolutely - but I think the fragility of the fcs in cell passage argues that rather than being inevitable, human insertion is still strongly likely to have been required. Even if a big pond is waiting at the top, the virus needs to somehow swim upstream in a cell passage setting.
The analysis that you did Jack, is worthy of a publication in a Virology or Genome journal. Clearly, the deniers of the lab-leak or gain-of-function research are running out of maneuvering room. The High-specificity of hACE2 to human receptors suggest a strong serial passage component in human cells. The likelihood of this kind of viral selection coming from the wild is just about nil.
"It is also informative that antibodies specific for the Bat-SCoV Spike protein neutralize Bat-SBRD, which expresses a chimeric Spike protein, but not SARS-CoV, indicating the existence of neutralizing antibodies that target portions of Spike outside the RBD. Importantly, these results suggest that hmAbs specific for SARS-CoV, and by inference the current panel of SARS-CoV vaccines, may provide significant protection against other SARS-like CoVs that emerge from zoonotic pools by natural recombination or are ** deliberately designed to cross species.** " . . .
"Paired with a greater availability of reagents and therapeutics, our studies represent an approach for rapid recovery and testing of newly identified pathogens, and which may improve public health preparedness and intervention strategies against natural or ** intentional zoonotic-human epidemics. **".
" The ability to design and recover pathogens reconstituted from synthesized cDNAs has the potential to overcome these obstacles by allowing studies of replication and pathogenesis without identification of reservoir species or ** [without] cultivation of primary isolates.**
We don't need no stinking isolates. OIOW - We can just make it up. From 2008 no less. And Fauci still walks free as the highest paid government employee.
I don't know how your mind jumped to the idea that I had anything against this author. Granted I gave no reason for my choice to unsubscribe. (Perhaps that is because I believe in personal choice!). The simple fact is I have overwhelmed with a great many subscriptions. Substack has a way of sneaking in five or six extra subscriptions when signing up for a chosen one. I was not vigilent. The welcome email has ( I have subsequently learned) an unsubscribe link but if I miss that and delete the newsletter I am stuck. (Learning curve) Unfortunately many the newsletters do not have an unsubscribe button at the bottom of each newsletter, only a link to upgrade to paid. Not ideal for managing one's inbox for sure!!! Still friends?
How do you see the ZC45/ZXC21 sequences fitting in with HKU-3-3, from a phylogenetic perspective?
My own inclination [perhaps I should point out that I'm part of DRASTIC] has been that the RaTg13/ZC45/ZXC21 genomes were intermediate constructs/consensus sequences along the development path towards SARS-CoV-2, with some elements being artificially inserted and some arising from serial passage.
I agree that the HKU-3-3 conclusions you're laying out here merit far more attention; I'm going to bring this up with Daoyu and some others because it seems perfectly in keeping with the notion that classified research was continuous in the years since 2005 -especially in the US, China and certain European labs.
Pradhan's "Uncanny" inserts have been deeply intertwined with Fauci's scientific censorship, and my primary area of research in 2022 has been the research ties concerning the various HIV/SARS homologous portions within their respective genomes. Thus, I'm very curious to see how this puzzle piece might fit.
If you happen to read this, I'd be very interested in discussing specific details of recent findings that may help connect some of the dots, via chrixey@protonmail.com.
Thinking this may be relevant. 2005:
https://registry.opendata.aws/ncbi-covid-19/
This is great. Skeptics of contamination sources effecting SARS-CoV-2 genome alignment & assembly have access to the read-level data and can fund their own bioinformatics analysis to back their claims of arbitrariness. De novo sequence assembly by independent teams using no reference for alignment should be sufficient evidence to convince them. https://registry.opendata.aws/ncbi-sra/
Not sure I have a mental model ready for all, lol, that but it struck me as rather relevant.
ralph baric is not a genius after all ... just copy and paste under protection of big uncle ...
I still think ultimate answers lie in the captive Uyghur population.
Why wouldn't an immoral bio researcher utilize this population if it was available to them?
Serial passage with a real disposable human population. Now occurring on a global scale!
And if we have learned nothing else, it is that morality among these researchers and their financiers left the building with Elvis.
" If they were growing them in Vero cells, serial passage without intent could have result in the furin cleavage site."
My read is that the authors are clarifying that their sequence was fcs-negative even before any serial cell passage. Vero-6 passage often results in the fcs dropping from the sequence (https://www.biorxiv.org/content/10.1101/2021.12.16.473063v2 https://www.nature.com/articles/s41586-021-03237-4 ) so a pre-passage sequence is needed to be sure the wild virus has no fcs.
I read it both ways; de novo evolution of the fcs would adapt the virus to human hACE2.
Absolutely - but I think the fragility of the fcs in cell passage argues that rather than being inevitable, human insertion is still strongly likely to have been required. Even if a big pond is waiting at the top, the virus needs to somehow swim upstream in a cell passage setting.
The analysis that you did Jack, is worthy of a publication in a Virology or Genome journal. Clearly, the deniers of the lab-leak or gain-of-function research are running out of maneuvering room. The High-specificity of hACE2 to human receptors suggest a strong serial passage component in human cells. The likelihood of this kind of viral selection coming from the wild is just about nil.
Thank you, Xavier.
Six miners got fatal pneumonia from the cave. It's a long story.
"It is also informative that antibodies specific for the Bat-SCoV Spike protein neutralize Bat-SBRD, which expresses a chimeric Spike protein, but not SARS-CoV, indicating the existence of neutralizing antibodies that target portions of Spike outside the RBD. Importantly, these results suggest that hmAbs specific for SARS-CoV, and by inference the current panel of SARS-CoV vaccines, may provide significant protection against other SARS-like CoVs that emerge from zoonotic pools by natural recombination or are ** deliberately designed to cross species.** " . . .
"Paired with a greater availability of reagents and therapeutics, our studies represent an approach for rapid recovery and testing of newly identified pathogens, and which may improve public health preparedness and intervention strategies against natural or ** intentional zoonotic-human epidemics. **".
" The ability to design and recover pathogens reconstituted from synthesized cDNAs has the potential to overcome these obstacles by allowing studies of replication and pathogenesis without identification of reservoir species or ** [without] cultivation of primary isolates.**
We don't need no stinking isolates. OIOW - We can just make it up. From 2008 no less. And Fauci still walks free as the highest paid government employee.
Where is the unsubscribe button. I don't remember subscribing and do not wish to. Tx
I'm honestly laughing at this comment. You can google how to unsubsribe to substack authors. you can look at substack's FAQ page.
All I see in your comment is: I don't want to know the truth. How do I keep my blindfold on?
This is one of the best substacks.
I don't know how your mind jumped to the idea that I had anything against this author. Granted I gave no reason for my choice to unsubscribe. (Perhaps that is because I believe in personal choice!). The simple fact is I have overwhelmed with a great many subscriptions. Substack has a way of sneaking in five or six extra subscriptions when signing up for a chosen one. I was not vigilent. The welcome email has ( I have subsequently learned) an unsubscribe link but if I miss that and delete the newsletter I am stuck. (Learning curve) Unfortunately many the newsletters do not have an unsubscribe button at the bottom of each newsletter, only a link to upgrade to paid. Not ideal for managing one's inbox for sure!!! Still friends?
FYI... You can mute threads easily! Thx
Hey Dr. Lyons-Weiler, have you seen this?
"Primate hemorrhagic fever-causing arteriviruses are poised for spillover to humans"
https://www.cell.com/action/showPdf?pii=S0092-8674(22)01194-1
published 9/30/22
Sounds kinda like Baric's paper from 2016:
"SARS-like WIV1-CoV poised for human emergence"
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4801244/
I had not seen this. I hope they are not so obsessed with how bad it could be they actually foster human-adapted arteriviruses.
PS Thank you.
Dr. Lyons-Weiler,
How do you see the ZC45/ZXC21 sequences fitting in with HKU-3-3, from a phylogenetic perspective?
My own inclination [perhaps I should point out that I'm part of DRASTIC] has been that the RaTg13/ZC45/ZXC21 genomes were intermediate constructs/consensus sequences along the development path towards SARS-CoV-2, with some elements being artificially inserted and some arising from serial passage.
I agree that the HKU-3-3 conclusions you're laying out here merit far more attention; I'm going to bring this up with Daoyu and some others because it seems perfectly in keeping with the notion that classified research was continuous in the years since 2005 -especially in the US, China and certain European labs.
Pradhan's "Uncanny" inserts have been deeply intertwined with Fauci's scientific censorship, and my primary area of research in 2022 has been the research ties concerning the various HIV/SARS homologous portions within their respective genomes. Thus, I'm very curious to see how this puzzle piece might fit.
If you happen to read this, I'd be very interested in discussing specific details of recent findings that may help connect some of the dots, via chrixey@protonmail.com.
The first Spike Protein patent was filed in 2002: Patent No. 6372224
They started research on this bioweapon in 1999.
Ralph Baric is the one who created Remsdevir with a death rate of 47% that is being prescribed in US hospitals to "fight" Covdi-19!!!