Ignore the "Fact Checker" Opinion Web Sites. Here's a Collection of Resources on Spike Protein Pathogenicity for Your Use. Add Your Own in the Comments.
I'm not a scientist, but I have been collecting these links. Sorry, I don't have time to format them properly.
“In the current study, we show that S protein alone can damage vascular endothelial cells (ECs) by downregulating ACE2 and consequently inhibiting mitochondrial function.”
SARS-CoV-2 Spike Protein Impairs Endothelial Function via Downregulation of ACE 2
Yuyang Lei, Jiao Zhang, Cara R. Schiavon, Ming He, Lili Chen, Hui Shen, Yichi Zhang, Qian Yin, Yoshitake Cho, Leonardo Andrade, Gerald S. Shadel, Mark Hepokoski, Ting Lei, Hongliang Wang, Jin Zhang, Jason X.-J. Yuan, Atul Malhotra, Uri Manor, Shengpeng Wang, Zu-Yi Yuan, John Y-J. Shyy See fewer authors
“In this paper, we have shown that the SARS-CoV-2 S1 RBD binds to a number of aggregation-prone, heparin binding proteins including Aβ, α-synuclein, tau, prion, and TDP-43 RRM. These interactions suggests that the heparin-binding site on the S1 protein might assist the binding of amyloid proteins to the viral surface and thus could initiate aggregation of these proteins and finally leads to neurodegeneration in brain.”
SARS-CoV-2 spike protein interactions with amyloidogenic proteins: Potential clues to neurodegeneration
“Key to our findings is the demonstration that S1 promotes loss of barrier integrity in an advanced 3D microfluidic model of the human BBB, a platform that more closely resembles the physiological conditions at this CNS interface. Evidence provided suggests that the SARS-CoV-2 spike proteins trigger a pro-inflammatory response on brain endothelial cells that may contribute to an altered state of BBB function.”
The SARS-CoV-2 spike protein alters barrier function in 2D static and 3D microfluidic in-vitro models of the human blood–brain barrier
“We conclude that SARS-CoV-2 spike glycoprotein, efficiently primed, activated and strategically poised during biosynthesis, can exploit the CM's inherent membranous connectivity to drive heart damage directly, uncoupling clinically common myocardial injury from lymphocytic myocarditis, often suspected but rarely confirmed in COVID-19.”
SARS-CoV-2 direct cardiac damage through spike-mediated cardiomyocyte fusion
Jay W. Schneider 1 # , David R. Pease 1 #, Chanakha K. Navaratnarajah 2 #, Peter Halfmann 3, Daniel J. Clemens 4, Dan Ye 4, 5, Chang Sung Kim 4, 5, Alison Barkhymer 2, Stephen Cohle 6, Aron Banks 7, Arpit Mehta 7, Joseph Rantus 7, Tim L. Emmerzaal 8, Tamás Kozicz 8, Kyle G. Howell 9, Jon E. Charlesworth 9, Trace A. Christensen 9, Yoshihiro Kawaoka 3, 10, Leslie T. Cooper 11, Michael J. Ackerman 4, 5, Roberto Cattaneo 2, and Wanek Family Program for HLHS-Stem Cell Pipeline
"We show a significant increase in the GC content of mRNAs in vaccines as compared to native SARS-CoV-2 RNA sequences encoding the spike protein. As the GC enrichment leads to more G-quadruplex structure formations, these may contribute to potential pathological processes initiated by SARS-CoV-2 molecular vaccination. “
My biggest question is related to myself. I am a Covid survivor (was not a big deal), never vaxxed.
Does the same spike protein negatively affect those who had symptomatic Covid? When I had my Covid infection, did the same Covid spikes damage my own immune system in the same way?
I had Covid a year ago, first day it was stomach flu, I was given IV fluids and stomach calming medications, then I had 4 more days of 101 fever and that was it.
I do not observe any immune system damage, so far I had two minor colds and that's it.
But I am somwehat worried that Covid virus and Covid spikes have the same deleterious effect as the vaccine free floating spike proteins.
I have been collecting studies since the beginning of the pandemic, so have quite a long list. While not exhaustive, I have been saving the most important ones (to me) in a goggle doc:
"There is an increased number of in vitro evidence that angioten-sin II (Ang II) may promote thrombosis
...
the contribution of coagulation and fibrinolytic systems in the mode of Ang II action was also determined. Venous thrombosis was induced by ligation of vena cava. Ang II infused into rats developing venous thrombosis caused dose-dependent increase in thrombus weight, which was partially reversed by losartan, selective AT1 antagonist. "
Paper Title: Angiotensin II enhances thrombosis development in renovascular hypertensive rats
One of the body's defenses against angiotensin, and forms of clotting, is ACEII. The virus and the jab expose the body to the spike protein. The spike protein attaches to ACEII and blocks the body's anti-inflammatory, anti-clotting response.
The drug Losartan reverses many of the inflammatory effects of spike, the jab, and the virus. Anecdotally, I've tested Losartan for over a year and felt no negative side effects. Losartan restored my sense of taste and smell immediately, and gave me new lungs. My endurance climbing hills, for example, is better now, than when I was a teenager. Losartan also restored my hearing to childhood levels, before the rock concerts.
The stupid reply to an article like this is that people believe that the vaccine spike protein is harmless because of the special measures (chef's secret sauce) taken by our benevolent Big Pharma saviors. That is the exact crap that the MSM floats. Pretty weak, but good enough for many dummies.
SARS-CoV-2 Requires Cholesterol for Viral Entry and
Pathological Syncytia Formation, Sanders et al. Elife. 2021 Apr 23;10:e65962. doi: 10.7554/eLife.65962 This quiet research is directed to understanding the pathology of SARS-CoV-2, but the results seem to me to be directly transferable to the mRNA vaccines. It shows that cells expressing full length spike protein on their surface fuse to other cells that express the ACE 2 receptor, eventually creating multicellular syncytia. I believe a similar process is involved in placenta formation. The authors draw attention to the pathology this might cause in lung tissue: "Here, we report that co-culture of human cells expressing the receptor ACE2 with cells expressing SARS-CoV-2 spike, results in synapse-like intercellular contacts that initiate cell-cell fusion, producing syncytia resembling those we identify in lungs of COVID-19 patients." Might this also explain some of the vascular effects of the vaccine? Possible impacts on reproductive tissues or placental tissue? Interestingly, membrane cholesterol was needed for cell fusion, and this was connected to better covid outcomes in patients taking statins.
SARS–CoV–2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro, Jiang et al.
"Here, by using an in vitro cell line, we report that the SARS–CoV–2 spike protein significantly inhibits DNA damage repair, which is required for effective V(D)J recombination in adaptive immunity. Mechanistically, we found that the spike protein localizes in the nucleus and inhibits DNA damage repair by impeding key DNA repair protein BRCA1 and 53BP1 recruitment to the damage site. Our findings reveal a potential molecular mechanism by which the spike protein might impede adaptive immunity and underscore the potential side effects of full-length spike-based vaccines."
Yowsa. Spike interferes with the development of adaptive immunity?
https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8538446/
https://www.ahajournals.org/doi/10.1161/circ.144.suppl_1.10712?fbclid=IwAR0_cE-XH7YqgFarUG263RvZMe4HGGXquHe_XhTmjREtfyiuZ2Vr9d1ZZQE
I'm not a scientist, but I have been collecting these links. Sorry, I don't have time to format them properly.
“In the current study, we show that S protein alone can damage vascular endothelial cells (ECs) by downregulating ACE2 and consequently inhibiting mitochondrial function.”
SARS-CoV-2 Spike Protein Impairs Endothelial Function via Downregulation of ACE 2
Yuyang Lei, Jiao Zhang, Cara R. Schiavon, Ming He, Lili Chen, Hui Shen, Yichi Zhang, Qian Yin, Yoshitake Cho, Leonardo Andrade, Gerald S. Shadel, Mark Hepokoski, Ting Lei, Hongliang Wang, Jin Zhang, Jason X.-J. Yuan, Atul Malhotra, Uri Manor, Shengpeng Wang, Zu-Yi Yuan, John Y-J. Shyy See fewer authors
Originally published31 Mar 2021
https://doi.org/10.1161/CIRCRESAHA.121.318902
Circulation Research. 2021;128:1323–1326
“In this paper, we have shown that the SARS-CoV-2 S1 RBD binds to a number of aggregation-prone, heparin binding proteins including Aβ, α-synuclein, tau, prion, and TDP-43 RRM. These interactions suggests that the heparin-binding site on the S1 protein might assist the binding of amyloid proteins to the viral surface and thus could initiate aggregation of these proteins and finally leads to neurodegeneration in brain.”
SARS-CoV-2 spike protein interactions with amyloidogenic proteins: Potential clues to neurodegeneration
Danish Idrees 1, Vijay Kumar 2
Affiliations expand
PMID: 33789211 PMCID: PMC7988450 DOI: 10.1016/j.bbrc.2021.03.100
https://pubmed.ncbi.nlm.nih.gov/33789211/
“Key to our findings is the demonstration that S1 promotes loss of barrier integrity in an advanced 3D microfluidic model of the human BBB, a platform that more closely resembles the physiological conditions at this CNS interface. Evidence provided suggests that the SARS-CoV-2 spike proteins trigger a pro-inflammatory response on brain endothelial cells that may contribute to an altered state of BBB function.”
The SARS-CoV-2 spike protein alters barrier function in 2D static and 3D microfluidic in-vitro models of the human blood–brain barrier
Author links open overlay panelTetyana P.BuzhdyganabBrandon J.DeOrecAbigailBaldwin-LeclaircTrent A.BullockabHannah M.McGaryaJana A.KhanaRoshanakRazmpouraJonathan F.HaleaPeter A.GaliecRaghavaPotulaabAllison M.AndrewsabServio H.Ramirez
https://www.sciencedirect.com/science/article/pii/S096999612030406X
Preprint:
“We conclude that SARS-CoV-2 spike glycoprotein, efficiently primed, activated and strategically poised during biosynthesis, can exploit the CM's inherent membranous connectivity to drive heart damage directly, uncoupling clinically common myocardial injury from lymphocytic myocarditis, often suspected but rarely confirmed in COVID-19.”
SARS-CoV-2 direct cardiac damage through spike-mediated cardiomyocyte fusion
Jay W. Schneider 1 # , David R. Pease 1 #, Chanakha K. Navaratnarajah 2 #, Peter Halfmann 3, Daniel J. Clemens 4, Dan Ye 4, 5, Chang Sung Kim 4, 5, Alison Barkhymer 2, Stephen Cohle 6, Aron Banks 7, Arpit Mehta 7, Joseph Rantus 7, Tim L. Emmerzaal 8, Tamás Kozicz 8, Kyle G. Howell 9, Jon E. Charlesworth 9, Trace A. Christensen 9, Yoshihiro Kawaoka 3, 10, Leslie T. Cooper 11, Michael J. Ackerman 4, 5, Roberto Cattaneo 2, and Wanek Family Program for HLHS-Stem Cell Pipeline
https://europepmc.org/article/ppr/ppr232448
Please be a bit more accurate and say "putting a mRNA that will encode the spike protein into the human body by injection"
****Preprint
Differences in Vaccine and SARS-CoV-2 Replication Derived mRNA: Implications for Cell Biology and Future Disease
Kevin McKernan1, Anthony M. Kyriakopoulos2, Peter McCullough3
https://www.researchgate.net/publication/356545068_Differences_in_Vaccine_and_SARS-CoV-2_Replication_Derived_mRNA_Implications_for_Cell_Biology_and_Future_Disease
"We show a significant increase in the GC content of mRNAs in vaccines as compared to native SARS-CoV-2 RNA sequences encoding the spike protein. As the GC enrichment leads to more G-quadruplex structure formations, these may contribute to potential pathological processes initiated by SARS-CoV-2 molecular vaccination. “
https://www.nejm.org/doi/full/10.1056/NEJMcibr2113694
Great article.
My biggest question is related to myself. I am a Covid survivor (was not a big deal), never vaxxed.
Does the same spike protein negatively affect those who had symptomatic Covid? When I had my Covid infection, did the same Covid spikes damage my own immune system in the same way?
I had Covid a year ago, first day it was stomach flu, I was given IV fluids and stomach calming medications, then I had 4 more days of 101 fever and that was it.
I do not observe any immune system damage, so far I had two minor colds and that's it.
But I am somwehat worried that Covid virus and Covid spikes have the same deleterious effect as the vaccine free floating spike proteins.
Any idea?
I have been collecting studies since the beginning of the pandemic, so have quite a long list. While not exhaustive, I have been saving the most important ones (to me) in a goggle doc:
https://docs.google.com/document/d/10qDWzHN9cfLeRkn3WvOVeVVY_t_zQgoMjUaalJezRPg/edit
https://www.thieme-connect.com/products/ejournals/abstract/10.1160/TH04-10-0701?fbclid=IwAR2Cxl9Cc2VHH17WZSf5_kWU7NHU1y_dX9EjhunKhfdxvL6SWpVq16VfiUI
"There is an increased number of in vitro evidence that angioten-sin II (Ang II) may promote thrombosis
...
the contribution of coagulation and fibrinolytic systems in the mode of Ang II action was also determined. Venous thrombosis was induced by ligation of vena cava. Ang II infused into rats developing venous thrombosis caused dose-dependent increase in thrombus weight, which was partially reversed by losartan, selective AT1 antagonist. "
Paper Title: Angiotensin II enhances thrombosis development in renovascular hypertensive rats
One of the body's defenses against angiotensin, and forms of clotting, is ACEII. The virus and the jab expose the body to the spike protein. The spike protein attaches to ACEII and blocks the body's anti-inflammatory, anti-clotting response.
The drug Losartan reverses many of the inflammatory effects of spike, the jab, and the virus. Anecdotally, I've tested Losartan for over a year and felt no negative side effects. Losartan restored my sense of taste and smell immediately, and gave me new lungs. My endurance climbing hills, for example, is better now, than when I was a teenager. Losartan also restored my hearing to childhood levels, before the rock concerts.
Thanks James. I got some good stuff. Lancet Letter Demolishes Vaccination
Says vaccination does not even slow down the pandemic. https://igorchudov.substack.com/p/lancet-article-demolishes-vaccination, https://www.thelancet.com/action/showPdf?pii=S2666-7762%2821%2900258-1
The stupid reply to an article like this is that people believe that the vaccine spike protein is harmless because of the special measures (chef's secret sauce) taken by our benevolent Big Pharma saviors. That is the exact crap that the MSM floats. Pretty weak, but good enough for many dummies.
SARS-CoV-2 Requires Cholesterol for Viral Entry and
Pathological Syncytia Formation, Sanders et al. Elife. 2021 Apr 23;10:e65962. doi: 10.7554/eLife.65962 This quiet research is directed to understanding the pathology of SARS-CoV-2, but the results seem to me to be directly transferable to the mRNA vaccines. It shows that cells expressing full length spike protein on their surface fuse to other cells that express the ACE 2 receptor, eventually creating multicellular syncytia. I believe a similar process is involved in placenta formation. The authors draw attention to the pathology this might cause in lung tissue: "Here, we report that co-culture of human cells expressing the receptor ACE2 with cells expressing SARS-CoV-2 spike, results in synapse-like intercellular contacts that initiate cell-cell fusion, producing syncytia resembling those we identify in lungs of COVID-19 patients." Might this also explain some of the vascular effects of the vaccine? Possible impacts on reproductive tissues or placental tissue? Interestingly, membrane cholesterol was needed for cell fusion, and this was connected to better covid outcomes in patients taking statins.
SARS–CoV–2 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro, Jiang et al.
"Here, by using an in vitro cell line, we report that the SARS–CoV–2 spike protein significantly inhibits DNA damage repair, which is required for effective V(D)J recombination in adaptive immunity. Mechanistically, we found that the spike protein localizes in the nucleus and inhibits DNA damage repair by impeding key DNA repair protein BRCA1 and 53BP1 recruitment to the damage site. Our findings reveal a potential molecular mechanism by which the spike protein might impede adaptive immunity and underscore the potential side effects of full-length spike-based vaccines."
Yowsa. Spike interferes with the development of adaptive immunity?